A. 3D ultrasound volume with CrystalVue™ and RealisticVue™ rendering. The volume was sectioned in a coronal plane to visualise internal structures.
B. Histological section of the matched specimen from the Carnegie Collection. A matching imaging plane was selected to validate visualised structures. The original specimen was sectioned in the transverse plane. The sections were imported in to a digital post-processing software (Amira, ThermoFisher Scientific) and aligned. From this imaging stack, the software can recreate other imaging planes, including the coronal imaging plane as shown in this image.
C. Annotated cross section of a 3D embryo model based on histological sections as shown in image B, the cross section was taken in the same imaging plane as image B.
D. Annotated 3D ultrasound volume based on comparison with the histological sections and 3D embryo models as shown in images B and C.
Image credit for images B and C: 3D Atlas of Human Embryology, B.S. de Bakker et al., An interactive three-dimensional digital atlas and quantative database of human develpoment, Science 2016; 354